Intracellular pH Changes of Starfish Sperm Upon the Acrosome Reaction. (acrosome reaction/intracellular pH/starfish sperm/egg jelly/9-aminoacridine)
نویسندگان
چکیده
منابع مشابه
Regulation of the starfish sperm acrosome reaction by cGMP, pH, cAMP and Ca2+.
In the starfish, Asterias amurensis, three components in the jelly coat of eggs, namely acrosome reaction-inducing substance (ARIS), Co-ARIS and asterosap, act in concert on homologous spermatozoa to induce the acrosome reaction (AR). Molecular recognition between the sperm surface molecules and the egg jelly molecules must underlie signal transduction events triggering the AR. Asterosap is a s...
متن کاملEgg sialoglycans increase intracellular pH and potentiate the acrosome reaction of sea urchin sperm.
Sea urchin egg jelly (EJ) triggers sperm acrosome reaction (AR), an exocytotic event required for membrane fusion of the gametes. Purified fucose sulfate polymer (FSP) in EJ is one inducer of the AR. Binding of FSP to its receptor regulates opening of two distinct calcium channels and also elevates intracellular pH (pH(i)). EJ also contains sialic acid-rich glycans (sialoglycans (SG)) that were...
متن کاملRegulatory mechanisms of the acrosome reaction revealed by multiview microscopy of single starfish sperm
The acrosome reaction in many animals is a coupled reaction involving an exocytotic step and a dramatic change in cell shape. It has been proposed that these morphological changes are regulated by intracellular ions such as Ca2+ and H+. We report here simultaneous visualization, under a multiview microscope, of intracellular free Ca2+ concentration ([Ca2+]i), intracellular pH (pHi), and morphol...
متن کاملSeparation of the sperm agglutinin and the acrosome reaction-inducing substance in egg jelly of starfish.
The egg jelly of the starfish Asterias amurensis was separated into the fractions J1, J2, and J3 on a Sephadex G-100 column. The Jl fraction induced the acrosome reaction and J2 induced sperm agglutination. Chemical analysis and chromatography revealed that sperm agglutinin is similar to asterosaponin A.
متن کاملThe Acrosome Reaction In
Suspensions of Mytilus edulis eggs were fixed with osmium tetroxide at various intervals between 1 and l0 seconds after heavy insemination, and sectioned for electron microscopy to follow the natural process of acrosome reaction in the spermatozoa around the eggs. Sperm suspensions were also fixed after the addition of I0 per cent by volume of M/3 calcium chloride. Within the first second after...
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ژورنال
عنوان ژورنال: Development, Growth and Differentiation
سال: 1986
ISSN: 0012-1592,1440-169X
DOI: 10.1111/j.1440-169x.1986.00359.x